ALCIAN BLUE-ALCIAN YELLOW: A NEW METHOD FOR THE IDENTIFICATION OF DIFFERENT ACIDIC GROUPS
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چکیده
منابع مشابه
Basophil counting with a new staining method using alcian blue.
Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspeci...
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The present paper describes a simple, sensitive technique for measurement of respiratory mucous glycoproteins that involves their precipitation with the cationic dye Alcian Blue. The assay was adapted from that described by Whiteman (1973) for measurement of urinary glycosaminoglycans. This method is useful for measurement of mucins secreted in response to stimulating agents in isolated airway ...
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Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then...
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Since Steedman ( 1950) has introduced it into histochemistry, alcian blue has been advocated to reveal a staining selectivity which depends upon the presence of acidic groups in mucopolysaccharides (Mowry, 1956; Pearse, 1960). As a plausible ground of this concept the fact has been presented that the alcian blue staining of polysaccharides can be increased in intensity, when acidic groups are i...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1964
ISSN: 0022-1554,1551-5044
DOI: 10.1177/12.1.44